Slide 3 of 16
Notes:
The first problem encountered in biological NMR spectroscopy concerns the native solvent: WATER.
The most informative nucleus is 1H (proton), however, water contains protons! In particular, solvent protons have a significantly higher concentration than the solutes. For a typical protein/DNA -sample that means: the protons of interest /the protein sample, are about 0.5 to 2 mM in concentration, water however, is 55M = 110 M in 1H. This water signal dominates the entire spectrum (10000 fold excess!); sample peaks are not or only barely visible.
