Slide 16 of 16
Notes:
- Why is water a problem for NMR
- Why do we work in water, not D2O
- What is the chemical shift time scale
- What is the difference between excitation/ non-excitation water supression techniques
- Do we see all protons in amino acids /nucleotides
- What is / was the preferred pH for NMR in water
- What kinetic range can NMR characterize
- Explain the temperature dependance of the HN-chemical shifts
- Explain the pH sensitivity of the HN-chemical shifts
