Biomolecular NMR-spectroscopy-Part II

Lecture 1: March 17



• NMR in water: the art of solvent supression
  • water concentration
  • signal digitization, gain
  • water removal by excitation
  • non-excitation filters: selective excitation
• Biological spin systems
  • amino acids,
  • nucleotides
  • in water / in D2O
• exchange kinetics: slow exchange to fast exchange
• random coil chemical shifts

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Recommended books, used in the preparation of these lectures:

Wüthrich, NMR of Proteins and Nucleic acids, Wiley NY1986, Chapter 2, pp. 13-25

Derome, Modern NMR Techniques for Chemical Research, Pergamon Press, 1987.
Sanders and Hunter, Modern NMR Spectroscopy, Oxford University Press, 1993.