2D- NMR resonance assignment

Table of Contents

2D- NMR resonance assignment

Spin type assignment

Spin system of the 20 amino acids

Chemical shift ranges of 1H-resonances

Chemical shift distributions

Categorization of spin systems based on knowledge and chemical shift

Example: DQF-COSY spectrum of vasopressin

Vasopressin - aliphatic region of the DQF-COSY

DQF-COSY fingerprint region

Vasopressin: Amide region of the TOCSY spectrum

Sequential assignment

Practical considerations:

Example: Vasopressin

Alternatively: Sequential assignment via amide proton NOEs

Information on bond angles from coupling constants

Typical NMR-data summary to highlight secondary structure

Email: frank@herring.phol.cwru.edu

Home Page: pout.phol.cwru.edu:8080/~frank/home.html

Other information:
Course notes Phol/Pharm/Biol 430 Lecture 4 March 31, 1998