Practical considerations:

To easily correlate residues sequentially, a direct comparison of the “fingerprint”regions of the NOESY and COSY spectrum works best. The task then is to connect COSY peaks (intra residue) with NOESY peaks (inter residue) in an alternate fashion. This leads to the following proton pathway:

N

H

C

O

Ca

H

N

H

C

O

Ca

H

N

H

C

O

Ca

H

R1

HCH

R2

HCH

R3

HCH

daN

C

N

C

N

C

N

dNa

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